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dcc  (R&D Systems)


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    Structured Review

    R&D Systems dcc
    Dcc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+dcc/Mouse+DCC+Antibody/pm41407191-360-4-5
    Average 93 stars, based on 26 article reviews
    dcc - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: A tug of war between DCC and ROBO1 signaling during commissural axon guidance.
    Article Snippet: After being cryopreserved in 30% sucrose, the samples were embedded in OCT (Optimal Cutting Temperature) and 20 mm thick sections were collected using a cryostat (CM3050s, Leica). .. Antibodies used for IHC are as follows: anti-DCC (AF844, R&D), anti-ROBO1 (AF1749, R&D), anti-ROBO2 (E4M6D, Cell Signaling Technology), anti-ROBO3,18 anti-ROBO3.1,26 anti-TAG1 (AF4439, R&D), anti-NF (2H3, DSHB), and Alexa Fluor 594-conjugated secondary antibodies (Jackson ImmunoResearch). ..

    Article Title: A tug of war between DCC and ROBO1 signaling during commissural axon guidance
    Article Snippet: After being cryopreserved in 30% sucrose, the samples were embedded in OCT (Optimal Cutting Temperature) and 20 μm thick sections were collected using a cryostat (CM3050s, Leica). .. Antibodies used for IHC are as follows: anti-DCC (AF844, R&D), anti-ROBO1 (AF1749, R&D), anti-ROBO2 (E4M6D, Cell Signaling Technology), anti-ROBO3, anti-ROBO3.1, anti-TAG1 (AF4439, R&D), anti-NF (2H3, DSHB), and Alexa Fluor 594-conjugated secondary antibodies (Jackson ImmunoResearch). .. After being cryopreserved in 30% sucrose, the samples were embedded in OCT (Optimal Cutting Temperature) and 20 μm thick sections were collected using a cryostat (CM3050s, Leica).


    Immunoprecipitation:

    Article Title: Regulation of Muscle Stem Cell Dynamics and Quiescence by Netrin-1 Cytoskeletal Signaling
    Article Snippet: .. Brain lysate was immunoprecipitated with anti-Dcc (R&D Systems, AF-844) and anti-Neo1 antibodies, then washed five times with lysis buffer. ..

    Lysis:

    Article Title: Regulation of Muscle Stem Cell Dynamics and Quiescence by Netrin-1 Cytoskeletal Signaling
    Article Snippet: .. Brain lysate was immunoprecipitated with anti-Dcc (R&D Systems, AF-844) and anti-Neo1 antibodies, then washed five times with lysis buffer. ..

    SDS Page:

    Article Title: Netrin-1 contributes to peripheral nerve injury induced neuropathic pain via regulating phosphatidylinositol 4-kinase IIa in the spinal cord dorsal horn in mice.
    Article Snippet: Background: The burden of neuropathic pain is growing worldwide.. Recent studies recapitulate the requirement for AMPA receptor in excitatory synaptic plasticity underlying pain-related syndromes.. Netrin-1 and its receptor deleted in colorectal cancer (DCC) are fundamental for AMPA receptor dependent synaptic transmission.

    Membrane:

    Article Title: Netrin-1 contributes to peripheral nerve injury induced neuropathic pain via regulating phosphatidylinositol 4-kinase IIa in the spinal cord dorsal horn in mice.
    Article Snippet: Background: The burden of neuropathic pain is growing worldwide.. Recent studies recapitulate the requirement for AMPA receptor in excitatory synaptic plasticity underlying pain-related syndromes.. Netrin-1 and its receptor deleted in colorectal cancer (DCC) are fundamental for AMPA receptor dependent synaptic transmission.

    Incubation:

    Article Title: Netrin-1 contributes to peripheral nerve injury induced neuropathic pain via regulating phosphatidylinositol 4-kinase IIa in the spinal cord dorsal horn in mice.
    Article Snippet: Background: The burden of neuropathic pain is growing worldwide.. Recent studies recapitulate the requirement for AMPA receptor in excitatory synaptic plasticity underlying pain-related syndromes.. Netrin-1 and its receptor deleted in colorectal cancer (DCC) are fundamental for AMPA receptor dependent synaptic transmission.

    Article Title: Netrin‐4 Modulates Corneal Wound Healing by Enhancing Corneal Epithelium and Nerve Regeneration
    Article Snippet: .. For this, freshly isolated TG neurons were incubated with the following concentrations of antibodies that should block > 90% of the concentration of Netrin‐4 used in our assays: 0.2 μg/mL anti‐DCC (AF844), 1.5 μg/mL anti‐Neogenin (AF1079), 0.5 μg/mL anti‐UNC5H1 (AF1405), or 0.5 μg/mL anti‐Integrin α6β1 (MAB78009) antibodies, all from R&D systems. ..

    Imaging:

    Article Title: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo
    Article Snippet: .. Following the first imaging session (0 h), 30 nl of vehicle solution (0.1 % BSA, 50 % Niu Twitty), recombinant chicken netrin-1 (300 ng/μl), rat UNC5H2 Fc chimera (UNC5H2-Ig; 300 ng/μl), function--blocking antibody to DCC (50 ng; GeneTex (Irvine, CA, USA), anti-DCC, AF5), or recombinant human IgG1 Fc (R&D Systems Inc., Minneapolis, MN, USA) was pressure injected both medially and laterally into the ventricle and the subpial space overlying the optic tectum. ..

    Recombinant:

    Article Title: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo
    Article Snippet: .. Following the first imaging session (0 h), 30 nl of vehicle solution (0.1 % BSA, 50 % Niu Twitty), recombinant chicken netrin-1 (300 ng/μl), rat UNC5H2 Fc chimera (UNC5H2-Ig; 300 ng/μl), function--blocking antibody to DCC (50 ng; GeneTex (Irvine, CA, USA), anti-DCC, AF5), or recombinant human IgG1 Fc (R&D Systems Inc., Minneapolis, MN, USA) was pressure injected both medially and laterally into the ventricle and the subpial space overlying the optic tectum. ..

    Droplet Countercurrent Chromatography:

    Article Title: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo
    Article Snippet: .. Following the first imaging session (0 h), 30 nl of vehicle solution (0.1 % BSA, 50 % Niu Twitty), recombinant chicken netrin-1 (300 ng/μl), rat UNC5H2 Fc chimera (UNC5H2-Ig; 300 ng/μl), function--blocking antibody to DCC (50 ng; GeneTex (Irvine, CA, USA), anti-DCC, AF5), or recombinant human IgG1 Fc (R&D Systems Inc., Minneapolis, MN, USA) was pressure injected both medially and laterally into the ventricle and the subpial space overlying the optic tectum. ..

    Injection:

    Article Title: Netrin-1 directs dendritic growth and connectivity of vertebrate central neurons in vivo
    Article Snippet: .. Following the first imaging session (0 h), 30 nl of vehicle solution (0.1 % BSA, 50 % Niu Twitty), recombinant chicken netrin-1 (300 ng/μl), rat UNC5H2 Fc chimera (UNC5H2-Ig; 300 ng/μl), function--blocking antibody to DCC (50 ng; GeneTex (Irvine, CA, USA), anti-DCC, AF5), or recombinant human IgG1 Fc (R&D Systems Inc., Minneapolis, MN, USA) was pressure injected both medially and laterally into the ventricle and the subpial space overlying the optic tectum. ..

    Isolation:

    Article Title: Netrin‐4 Modulates Corneal Wound Healing by Enhancing Corneal Epithelium and Nerve Regeneration
    Article Snippet: .. For this, freshly isolated TG neurons were incubated with the following concentrations of antibodies that should block > 90% of the concentration of Netrin‐4 used in our assays: 0.2 μg/mL anti‐DCC (AF844), 1.5 μg/mL anti‐Neogenin (AF1079), 0.5 μg/mL anti‐UNC5H1 (AF1405), or 0.5 μg/mL anti‐Integrin α6β1 (MAB78009) antibodies, all from R&D systems. ..

    Blocking Assay:

    Article Title: Netrin‐4 Modulates Corneal Wound Healing by Enhancing Corneal Epithelium and Nerve Regeneration
    Article Snippet: .. For this, freshly isolated TG neurons were incubated with the following concentrations of antibodies that should block > 90% of the concentration of Netrin‐4 used in our assays: 0.2 μg/mL anti‐DCC (AF844), 1.5 μg/mL anti‐Neogenin (AF1079), 0.5 μg/mL anti‐UNC5H1 (AF1405), or 0.5 μg/mL anti‐Integrin α6β1 (MAB78009) antibodies, all from R&D systems. ..

    Article Title: Paracrine Pax6 activity regulates oligodendrocyte precursor cell migration in the chick embryonic neural tube.
    Article Snippet: Secondary antibodies were either Alexa Fluor 594 or Alexa Fluor 488 immunoglobulin (Molecular Probes). .. The blocking antibodies used were polyclonal anti-Pax6 (rabbit; 10 g/ml Covance), monoclonal anti-Pax6 (mouse; 100 g/ml, DSHB), monoclonal anti-Engrailed (mouse; 100 g/ml; DSHB), anti-DCC (polyclonal; goat; 20 g/ml, R&D Systems) and anti-netrin (polyclonal, goat; 20 g/ml, R&D Systems). ..

    Concentration Assay:

    Article Title: Netrin‐4 Modulates Corneal Wound Healing by Enhancing Corneal Epithelium and Nerve Regeneration
    Article Snippet: .. For this, freshly isolated TG neurons were incubated with the following concentrations of antibodies that should block > 90% of the concentration of Netrin‐4 used in our assays: 0.2 μg/mL anti‐DCC (AF844), 1.5 μg/mL anti‐Neogenin (AF1079), 0.5 μg/mL anti‐UNC5H1 (AF1405), or 0.5 μg/mL anti‐Integrin α6β1 (MAB78009) antibodies, all from R&D systems. ..



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